| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
FISH Microdeletion
Test Code510388
Alias/See Also
Microdeletion Syndrome Analysis, Fluorescence in situ Hybridization (FISH)
Preferred Specimen
Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei for each probe indicated, blood, bone marrow, amniotic fluid, chorionic villi/products of conception or slides from buccal smears
Minimum Volume
Fixed-cell pellet from a cytogenetic analysis, two slides, 5 mL blood (adult), 1 mL blood (pediatric), 1 mL bone marrow, or 5 mL amniotic fluid
Instructions
When buccal smears are used to make slides, follow these When buccal smears are used to make slides, follow these Have the patient rinse the oral cavity twice with drinking water. Scrape the inside of the oral cavity well using only one side of the premoistened appilcator. A cotton swab can be used if applicator is not available. Discard first scraping and repeat with slightly more pressure. Smear the collected specimen gently and evenly across a prelabeled glass slide. Allow the slide to air-dry at room temperature for 10 to 30 minutes. Check the quality of the slide with phase microscopy, if available, before proceeding. A good slide has 30-100 cells/lpf and appropriate morphology (ie, lying flat, no residue or film, not refractile, gray in color). Dip the slide(s) in a Coplin jar with 50% methanol/50% acetic acid for ten minutes (or spray with a cytology spray)and air-dry.
Transport Container
All blood or bone marrow in sodium heparin tube (pediatric Vacutainer(R) is optimal), confluent T-25 flask (amniotic fluid)
Transport Temperature
Room Temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Broken or dirty slides; excessive cellular debris; stains on slides
Methodology
Fluorescence in situ hybridization (FISH)
Setup Schedule
Daily (1,2)
