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11-DESOXYCORTISOL
Message"No isotopes administered 24 hours prior to venipuncture
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Test Code
500550
Alias/See Also
"11-Desoxycortisol
Compound S for Metyrapone Test
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Compound S for Metyrapone Test
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CPT Codes
82634
Preferred Specimen
"Serum
0.5 mL
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0.5 mL
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Minimum Volume
"0.2 mL (Note: This volume does not allow for repeat testing.)
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Instructions
"Transfer the serum into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp N° 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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Transport Container
"Red-top tube or gel-barrier tube
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Transport Temperature
Frozen
Specimen Stability
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Room temperature---1 day
Refrigerated---1 day
Frozen---2 years
Freeze/thaw cycles---Stable x3
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Room temperature---1 day
Refrigerated---1 day
Frozen---2 years
Freeze/thaw cycles---Stable x3
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Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Recently administered isotopes
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Methodology
"Liquid chromatography/tandem mass spectrometry (LC/MS-MS) after chromatography and solvent extraction "
Clinical Significance
"Evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve
11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol. The conversion of 11-deoxycortisol to cortisol is inhibited by metyrapone, which acts on 11-ß-hydroxylase. The metyrapone test (see the online Endocrine Appendix: ACTH Stimulation) serves as a reliable and sensitive indicator of pituitary ACTH secretory reserve. The 11-deoxycortisol levels normally increase to 100 times the control value following metyrapone administration. Reduced response occurs in patients with hypoadrenalism or with hypopituitarism and in some patients with diseases of the hypothalamus. Patients with myxedema, some pregnant patients, and those on oral contraceptives respond poorly.
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11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol. The conversion of 11-deoxycortisol to cortisol is inhibited by metyrapone, which acts on 11-ß-hydroxylase. The metyrapone test (see the online Endocrine Appendix: ACTH Stimulation) serves as a reliable and sensitive indicator of pituitary ACTH secretory reserve. The 11-deoxycortisol levels normally increase to 100 times the control value following metyrapone administration. Reduced response occurs in patients with hypoadrenalism or with hypopituitarism and in some patients with diseases of the hypothalamus. Patients with myxedema, some pregnant patients, and those on oral contraceptives respond poorly.
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