Factor VIII Antigen

Message
Testing performed at Esoterix Coagulation Lab UY#300208


Test Code
500196


Alias/See Also
FVIII quantitation (non functional)


CPT Codes
83520

Preferred Specimen
Plasma, FROZEN


Minimum Volume
1 mL


Instructions
Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.[1] Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.[2,3] The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.[4,5] When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Serum gel tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge and carefully remove the plasma without disturbing the cells using a plastic transfer pipette. Transfer the plasma into a LabCorp plastic purple tube with cap (LabCorp ID 9566589372). The specimen should be FROZEN immediately and maintained frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Transport Container
Blue-top (sodium citrate) tube


Transport Temperature
FREEZE Stability: Room temperature up to 4 hours Refrigerate up to 8 hours Acceptable freeze/thaw up to 3 cycles


Methodology
Enzyme Linked Immunosorbent Assay (ELISA)



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.