FACTOR VIII ANTIGEN

Message
"Do not draw from an arm with a heparin lock or heparinized catheter
"


Test Code
500196


Alias/See Also
FVIII Quantitation (Nonfunctional)


CPT Codes
83520

Preferred Specimen
"Plasma
2 mL
"


Minimum Volume
"1 mL
"


Instructions
"Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge and carefully remove the plasma using a plastic transfer pipette, being careful not to disturb the cells. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp N° 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume."


Transport Container
"Blue-top (sodium citrate) tube
"


Transport Temperature
Frozen


Specimen Stability
"Freeze; up to three freeze/thaw cycles are acceptable. Stable at room temperature for up to four hours or refrigerated for up to eight hours.
"


Methodology
"Enzyme-linked immunosorbent assay (ELISA) "

Reference Range
"50% to 160%
"


Clinical Significance
"Quantitate the amount of factor VIII protein; not a measure of von Willebrand factor antigen (previously called FVIII-related antigen)
"




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.