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B-Cell Gene Rearrangement, Southern Blot
Message"This assay can be used to detect clonal B-cell rearrangements in blood, bone marrow, and tissue specimens. The presence of monoclonal gene rearrangement usually, but not always, reflects the presence of a B-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive conditions. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders.
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Test Code
480715
Alias/See Also
Immunoglobulin Heavy Chain Gene Rearrangement
CPT Codes
81262
Includes
"Please direct any questions regarding this test to oncology customer service at 800-345-4363.
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Preferred Specimen
"Whole blood, bone marrow, fresh or frozen tissue
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Minimum Volume
"3 mL whole blood
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Instructions
"Specimen should arrive in the laboratory within 48 hours of collection. Please state the date and time of collection on the request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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Transport Container
"Lavender-top (EDTA) tube, yellow-top (ACD-A) tube, or tissue in lymph node transport bottle
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Transport Temperature
"Maintain specimen at room temperature. Some specimen types may be submitted frozen; call 800-345-4363 for instructions.
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Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Specimen more than 48 hours old; clotted blood
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Methodology
"Southern blot analysis "
Limitations
"The Southern blot analysis may not detect a clonal population if it represents <10% of the total cells in the specimen. The B-Cell Gene Rearrangement, PCR [480716] test can be used to detect smaller populations of malignant cells in blood, bone marrow, and tissue (both fresh and paraffin-embedded).
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