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K-ras Gene Mutation Detection With Reflex to BRAF Gene Mutation Detection
Test Code480360
CPT Codes
81275
Preferred Specimen
"Formalin-fixed, paraffin-embedded tissue (FFPE)
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Minimum Volume
"2 mm x 2 mm tumor area
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Instructions
"Maintain blocks and slides at room temperature
Please provide five unstained slides and one H&E stained slide at 10 µM or a tissue block.
Please provide a copy of the pathology report. Please direct any questions regarding this test to customer service at 800-345-4363.
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Please provide five unstained slides and one H&E stained slide at 10 µM or a tissue block.
Please provide a copy of the pathology report. Please direct any questions regarding this test to customer service at 800-345-4363.
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Transport Container
"Slides, blocks
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Methodology
"Amplification refractory mutation system (ARMS) and real-time polymerase chain reaction using Scorpions™ technology "
Limitations
"The provided tumor tissue should be composed of =50% tumor cells for accurate test interpretation. Preparation of DNA from tissue samples is dependent upon the quality of the provided specimen. Inadequate DNA extraction may occur in a significant number of paraffin-embedded samples.
The methods used in this assay are highly selective and, depending on the total amount of DNA present, can detect approximately 1% of mutant in a background of wild-type genomic DNA. The assays have limits of detection of between 5 and 10 copies.
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The methods used in this assay are highly selective and, depending on the total amount of DNA present, can detect approximately 1% of mutant in a background of wild-type genomic DNA. The assays have limits of detection of between 5 and 10 copies.
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