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Barbiturate Screen and Confirmation, Urine
Message"The presence of barbiturates in urine is presumptively positive at a level >300 ng/mL using secobarbital as a standard and can indicate prescribed or abused intake of this class of drugs.
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Test Code
74419
Alias/See Also
"Amobarbital
Butalbital
Pentobarbital
Phenobarbital
Secobarbital"
Butalbital
Pentobarbital
Phenobarbital
Secobarbital"
CPT Codes
80101
Includes
"Identification and confirmation of barbiturates in urine. Confirmation by GC/MS is automatically performed on positive samples for additional charge.
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Preferred Specimen
"Urine
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Minimum Volume
"30 mL
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Instructions
"Chain-of-custody documentation is required for samples submitted for preëmployment, random employee testing, and forensic purposes. For other applications, use the standard request form. Please mark chain-of-custody test number on the request form.
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Transport Container
"Use plastic urine drug bottle and tamper-evident seal for forensic specimen. Collection kits are available by request from the laboratory.
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Transport Temperature
"Urine temperature monitoring is recommended for samples to be tested for medicolegal purposes.
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Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Quantity not sufficient for analysis; improper specimen (serum, plasma, blood); incomplete chain-of-custody documentation; incomplete specimen identification; improper or missing tamper-evident seals
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Methodology
"Initial testing by immunoassay; confirmation of positives by gas chromatography/mass spectrometry (GC/MS) "
Limitations
"Short- and intermediate-acting barbiturates can be detected in urine for at least 24 to 72 hours following ingestion, longer-acting drugs for at least seven days.
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Clinical Significance
"Urine drugs of abuse testing
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