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Intrinsic Pathway Factors
Test Code500033
CPT Codes
85240, 85250, 85270, 85280
Preferred Specimen
Plasma, FROZEN
Minimum Volume
2mL
Instructions
Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.[1] Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.[2,3] The sample should be mixed immediately by gentle inversion at least 6 times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.[4,5] When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier gel tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge and (using a plastic transfer pipette) carefully remove the plasma without disturbing the cells. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp ID 23751). The specimen should be FROZEN immediately and main- tained frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Transport Container
Blue-top (sodium citrate) tube
Transport Temperature
FREEZE
Methodology
See individual test descriptions