| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
VIRAL CULTURE,RAPID,LESION
Test Code186056
CPT Codes
87254; 87255
Includes
Cell culture by ELVIS® method; identification and typing of herpes simplex virus. Shell vials or equivalent multiwell plate culture with fluorescent antibody staining for varicella-zoster. CPT coding for microbiology and virology procedures often cannot b
Preferred Specimen
Vesicular fluid, ulcerated lesions, vesicular scrapings, pharyngeal and throat swabs, autopsy and biopsy material, vaginal swabs
Minimum Volume
Swab in viral transport media, 0.5 g tissue in viral transport medium
Instructions
"Specimen is best collected within the first three days after appearance of lesion, but no longer than seven days. Do not prepare the collection site with alcohol or iodophors. After collection, remove cap from the transport media tube and insert swab aseptically into transport tube and break swab shaft. Replace cap so that swab will not interfere with closure and allow tube to leak. Label tube with patient identification and transport to the laboratory at 4°C.
Submit one specimen per test requested. Specify the exact specimen source/origin (eg, genital lesion). Indicate a specific test number on the test request form. Do not use transport device beyond the expiration date."
Submit one specimen per test requested. Specify the exact specimen source/origin (eg, genital lesion). Indicate a specific test number on the test request form. Do not use transport device beyond the expiration date."
Transport Container
"Viral, Chlamydia, or Mycoplasma culture transport provided by LabCorp, or other appropriate transport medium; sterile screw-cap container
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Transport Temperature
Specimen should be kept at 4°C (refrigeration) and transported to the laboratory within 24 hours of collection.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Bacterial swab specimen; specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additive; specimen received in expired transport media or incorrect transport device; inappropriate specimen transport cond
Methodology
"Enzyme-linked virus-inducible system (ELVIS®). ELVIS® HSV was developed using a transgenic baby hamster kidney-21 cell line. In this cell line, HSV infection causes the expression of a beta-galactosidase “reporter gene,” and it is the expression of this gene that can be used to monitor infection by HSV. Cultures positive by ELVIS® are confirmed by immunofluorescent staining. Shell vials or equivalent multiwell plate culture with fluorescent antibody staining is utilized for the recovery of varicella-zoster. "
