QuantiFERON Client Incubated

This test is time-sensitive and specimens must be received in the laboratory within 70 hours after incubation by the client (no more than 94 hours from the time of collection). Specimen collection times will vary depending on logistics from the incubating

183244

Interferon-gamma Release Assay (IGRA) for Mycobacterium tuberculosis

Whole blood

0.8 mL x three tubes

Refer to collection instructions included with draw kit. Special specimen collection kit contains three gel-barrier tubes as noted above. All three tubes are required for a single test result. Each tube is designed to draw only 1 mL and fill time may be longer than other blood collection tubes. Because of the limited vacuum in these tubes, use a needle and holder (NOT a butterfly) to collect QuantiFERON(R) specimens. If a butterfly is required, first collect other required tubes or use another Vacutainer(R) tube to purge the butterfly line of air and then proceed with drawing the QuantiFERON(R) tubes. Fill tubes to the black fill line on the tube. If a tube is underfilled or overfilled (see kit insert), immediately collect replacement tube. Following proper fill, label the tubes appropriately and shake tubes 10 times firmly enough to ensure the entire surface of the tube is coated with blood cells to solubilize the antigen on the tube walls. After shaking, the volume may fall below the fill line. Do NOT centrifuge or refrigerate specimens. Return each of the three properly filled, labeled, and shaken tubes to the box labeled "QFT(R) kit". Seal the top by removing tape from the adhesive. Tubes should be incubated (in an incubator, not a water bath or heat block) upright for 16-24 hours at 37 degrees C +/- 1 degree C in the box/kit within 16 hours of collection. Please indicate date and time of venipuncture on the test request form. If the specimen has been incubated for 16-24 hours at 37 degrees C +/- 1 degree C, please mark the top of the specimen box where indicated to denote incubation. INCUBATION: If blood is not incubated immediately after collection, remix the tubes by inverting 10 times immediately prior to incubation and within 16 hours of collection. Within 16 hours of collection, incubate the tubes upright in an incubator at 37 degrees C +/- 1 degree C for 16 to 24 hours. The incubator does not require CO2 or humidification. Water baths or heat blocks are not suitable alternatives for a laboratory incubator. Following incubation, forward the tubes to the laboratory at 17 to 27 degrees C.

The QuantiFERON(R) collection kit contains the QuantiFERON (R) blood tubes and instructions for the collection and handling of (1) gray-top (with white ring), uncoated (nil); (2) red-top (with white ring), TB antigen-coated; (3) purple-top (with white rin

After incubation, maintain specimen at room temperature (17 to 27 degrees C). Do NOT centrifuge, refrigerate, or ship tubes on ice.

Specimen frozen; specimen more than 94 hours old on receipt by lab; unlabeled specimens not in QuantiFERON kit box.

M-TH (1,2,3) F/SAT (1,2) SUN (2,3)