Bacterial Vaginosis (Sialidase), Trichomonas vaginalis, NAA, Vaginal Yeast Culture

"Bradshaw CS, Morton AN, Garland SM, et al, “Evaluation of a Point-of-Care Test, BVBlue, and Clinical Laboratory Criteria for Diagnosis of Bacterial Vaginosis,” J Clin Microbiol, 2005, 43(3):1304-8.PubMed 15750100

Garcia LS, Diagnostic Medical Parasitology, 4th ed, Washington, DC: ASM Press, 2001.

Leber AL, Novak SM, “Intestinal and Urogenital Amebae, Flagellates, and Ciliates,” Manual of Clinical Microbiology, 7th ed, Washington, DC: ASM Press, 1999.

Rein MF, Trichomonas vaginalis, Principles and Practice of Infectious Diseases, 5th ed, Mandell GL, Bennett JE, Dolin R, eds, Philadelphia, PA: Churchill Livingston, 2000.

Sorvillo F, Smith L, Kerndt P, et al, “Trichomonas vaginalis, HIV, and African-Americans,” Emerg Infect Dis, 2001, 7(6):927-32.PubMed 11747718

Van Der Schee C, van Belkum A, Zwijers L, et al, “Improved Diagnosis of Trichomonas vaginalis Infection by PCR Using Vaginal Swabs and Urine Specimen Compared to Diagnosis by Wet Mount Microscopy, Culture, and Fluorescent Staining,” J Clin Micro, 1999, 37(12):4127-30.PubMed 10565943
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182717

BV (Sialidase), Tv (NAA), Vaginal Yeast (Culture)

"Vaginal fluid
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"Multiple swab specimens
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"Using transport swabs provided, collect vaginal fluid sample by contacting the lower one-third of the vaginal wall, rotating for 10 to 20 seconds to absorb fluid. Immediately place the swabs into the transport tubes. For the APTIMA® swab, carefully break the swab shaft against the side of the tube. Tightly screw on the cap.
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"Copan double swab in Aimes liquid medium and APTIMA® unisex swab transport
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"Maintain specimen at room temperature or refrigerate (2°C to 30°C).
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"Specimen collected or transport other than previously described; unlabeled specimens or those with a discrepancy between the specimen label and the request form; APTIMA® Combo 2 (AC2) swab specimen transport tube with two swabs or swab not supplied by Ge

"BV (sialidase) activity: Collecting specimens from the cervix should be avoided because (a) it might increase the risk to OB patients, and (b) cervical sialidase activity is usually higher than vaginal sialidase activity. Do not collect specimens from patients who have (a) used a vaginal cream or ointment product, (b) douched, or (c) used spermicides, vaginal lubricants, or feminine sprays within 72 hours of testing. If insufficient sample is collected or collected from a patient undergoing antimicrobial therapy, the test may yield a false-negative result.
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"BV (sialidase) activity: Enzyme activity test for use in the detection of vaginal fluid specimens for sialidase activity, an enzyme produced by bacteria associated with bacterial vaginitis, including Bacteroides spp, Prevotella spp, and Mobiluncus spp, and some G vaginalis. In a recent study, Bradshaw et al demonstrated the test to be 88% sensitive and 95% specific.

Vaginal yeast culture: Detect yeast in vaginal fluid

Trichomonas vaginalis by NAA: Diagnosis of T vaginalis infection
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