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Bacterial Vaginosis, Sialidase Activity
Message"Bradshaw CS, Morton AN, Garland SM, et al, “Evaluation of a Point-of-Care Test, BVBlue, and Clinical Laboratory Criteria for Diagnosis of Bacterial Vaginosis,” J Clin Microbiol, 2005, 43(3):1304-8.PubMed 15750100
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Test Code
180034
Alias/See Also
"BV (Sialidase) Activity
Vaginitis
Vaginosis"
Vaginitis
Vaginosis"
CPT Codes
87905
Preferred Specimen
"Vaginal fluid
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Minimum Volume
"Swab
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Instructions
"Using transport swabs provided, obtain a sample by contacting the lower one-third of the vaginal wall, rotating 10-20 seconds to absorb fluid. Immediately place the swabs into the transport tube.
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Transport Container
"Copan double swab in Amies liquid medium
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Transport Temperature
"Maintain specimen at room temperature or refrigerate for up to 72 hours.
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Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Specimen collected or transported other than previously described
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Methodology
"Chromogenic enzyme activity test "
Limitations
"Collecting specimens from the cervix should be avoided because (a) it might increase risk to OB patients, and (b) cervical sialidase activity is usually higher than vaginal sialidase activity. Do not collect specimens from patients who have (a) used a vaginal cream or ointment product, (b) douched, or (c) used spermicides, vaginal lubricants, or feminine sprays within 72 hours of testing. If insufficient sample is collected or collected from a patient undergoing antimicrobial therapy, the test may give a false-negative result.
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Clinical Significance
"Enzyme activity test for use in the detection of vaginal fluid specimens for sialidase activity, an enzyme produced by bacteria associated with bacterial vaginitis, including Bacteroides spp, Prevotella spp, and Mobiluncus spp and some G vaginalis. In a recent study, Bradshaw et al demonstrated the test to be 88% sensitive and 95% specific.
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