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HLA CLASS 1,2 ANTIBODY SCREEN
Message"Collect specimen prior to dialysis. Do not collect if patient has received antithymocyte globulin, OKT3, or alemtuzumab (Campath®) recently. These drugs may cause false-positive results.
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Test Code
168050
Alias/See Also
"HLA Antibody, PRA
HLA Class 1/2 Antibody, White Cell Antibody
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HLA Class 1/2 Antibody, White Cell Antibody
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CPT Codes
86828
Preferred Specimen
"Serum
7 mL
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7 mL
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Minimum Volume
"3 mL
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Instructions
"To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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Transport Container
"Red-tube top or gel-barrier tube
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Transport Temperature
Room temp
Specimen Stability
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Room temperature---2 days
Refrigerated---1 month
Frozen---2 years
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Room temperature---2 days
Refrigerated---1 month
Frozen---2 years
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Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Highly lipemic or hemolyzed specimen
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Methodology
"Solid phase (Luminex) "
Limitations
"This test provides limited information as to the HLA specificities of the antibodies. Detects antibodies of the IgG isotype and not IgM antibodies, which are usually indicative of autoantibodies.
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Clinical Significance
"Solid organ transplantation
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