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HSV 1/2 PCR
Message"HSV PCR is a single test that both detects the presence of HSV DNA and determines which type is present in the positive samples. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2.
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Test Code
138651
Alias/See Also
HSV Types 1 and 2, DNA PCR (Real Time)
CPT Codes
87529(x2)
Preferred Specimen
"Cerebrospinal fluid (CSF), swab, whole blood, serum, plasma, or vitreous fluid
0.5 mL CSF or vitreous fluid; one swab; or 1 mL whole blood, serum, or plasma
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0.5 mL CSF or vitreous fluid; one swab; or 1 mL whole blood, serum, or plasma
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Minimum Volume
"0.2 mL CSF or vitreous fluid; one swab; or 0.5 mL whole blood, serum, or plasma
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Instructions
"To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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Transport Container
"Sterile container (CSF or vitreous fluid), universal transport medium (swab), red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or yellow-top (ACD) tube
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Specimen Stability
"Whole blood is stable at room temperature or refrigerated for seven days; do not freeze. Swab samples are stable at room temperature, refrigerated, or frozen for seven days. Serum, plasma, or CSF samples are stable at room temperature or refrigerated for
Methodology
"Real-time polymerase chain reaction (PCR) "
Clinical Significance
"This test is intended for use as an aid in the diagnosis of herpes simplex virus (HSV) infections; it also differentiates HSV-1 from HSV-2. PCR testing of blood, serum, or plasma samples is clinically useful only in potential cases of disseminated HSV infection (neonates, immunosuppressed individuals) and not as an aid in the diagnosis of either mucosal or CNS disease.
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