LBC+PG, Amniotic Fluid

Test Code

120715

Alias/See Also

FLM; PG

CPT Codes
83664, 84081

Preferred Specimen

LBC: Amniotic fluid PG: Amniotic fluid, FROZEN

Minimum Volume

LBC: 1 mL PG: 0.2 mL

Instructions

LBC: Amniotic fluid is obtained by needle aspiration into the amniotic sac(amniocentesis). Do NOT centrifuge. Do NOT vortex. Do NOT freeze. NOTE: When LBC is ordered in panels or additional testing for the amniotic fluid is requested, special care must be taken to mix the samples thoroughly before splitting (or pouring off) the sample for additional testing. See below: * Mix the amniotic fluid by gentle inversion a minimum of 20 times (do NOT vortex). * Aspirate the sample with a clean, disposable pipette or pour the sample over into designated containers. * Label containers carefully as to sample storage and type: 1) amniotic fluid, refrigerated OR 2) amniotic fluid, FROZEN OR 3) amniotic fluid, spun-frozen, etc This will ensure the correct sample type is tested for appropriate analysis without the risk of compromising quality due to preanalytical errors. . PG: Centrifuge fluid at low speed (500xg) for three minutes. Carefully pour off the supernatant into a labeled transport tube. Discard the original tube and pellet. NOTE: Any prolonged centrifugation step can reduce recovery of the phospholipids to <50%. Amniotic fluid contaminated with blood meconium or vaginal secretion CAN be used for a PG determination but NOT LBC. To avoid delays in turnaround time when requesting multiple tests on frozen samples, PLEASE SUBMIT SEPARATE FROZEN SPECIMENS FOR EACH TEST REQUESTED.

Transport Container

LBC: Sterile container, no additive PG: Plastic transport tube

Transport Temperature

LBC: Refrigerate specimen upon collection. Do NOT freeze. Do NOT centrifuge. Do NOT vortex. PG: FREEZE within one hour to transport to laboratory. Avoid repeated freezing and thawing of the specimen, which may cause the sample to precipitate, resulting in

Specimen Stability

Room temp:LBC: up to 72 hours; PG: up to 1 hour Refrigerated: LBC: up to 14 days; PG: up to 4 hours Frozen: LBC: UNSTABLE; PG: up to 1 week

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

LBC: Frozen samples; bloody (RBC value >0.02 x 10E6/uL) or contaminated samples; large, gelatinous object(s) that will not aspirate; vaginal pools containing mucus; centrifuged specimens; vortexed specimens; specimens containing meconium; room temperatur

Methodology
LBC: Optical impedance; PG: Immunologic agglutination

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.