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B.PERTUSSISB.PARAPERTUSSIS PCR
Test Code138677
Alias/See Also
"B pertussis and B parapertussis, DNA PCR (Real-time)
Whooping Cough
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Whooping Cough
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CPT Codes
87798(x2)
Preferred Specimen
"Nasopharyngeal (NP) swab, throat swab (dacron or rayon), or nasopharyngeal aspirate/wash
One NP/throat swab or 0.5 mL nasal aspirate/wash
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One NP/throat swab or 0.5 mL nasal aspirate/wash
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Transport Container
"• Option 1 (preferred): Flocked NP swab submitted dry in plastic transport sleeve (PeopleSoft N° 93307)
• Option 2 (alternate): NP swab submitted in charcoal-containing transport media
• Option 3 (alternate): NP/throat swab submitted in universal trans
• Option 2 (alternate): NP swab submitted in charcoal-containing transport media
• Option 3 (alternate): NP/throat swab submitted in universal trans
Transport Temperature
"Maintain specimen at room temperature or refrigerate. Do not freeze charcoal transport swabs or dry swabs. Swabs in UTM and NP aspirates/washes may be frozen for up to 7 days.
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Specimen Stability
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Room temperature
5 days
Refrigerated
7 days
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Room temperature
5 days
Refrigerated
7 days
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Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Calcium alginate swabs; swabs in noncharcoal-containing transport media; swabs in Regan-Lowe culture media
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Methodology
"Real-time polymerase chain reaction (PCR) "
Limitations
"The target for the B pertussis PCR reaction, a region of IS481, is also found in Bordetella holmesii. A false-positive result for B pertussis DNA may occur if B holmesii is present in the sample tested.
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Clinical Significance
"This assay is intended to be used as an aid to the diagnosis of Bordetella pertussis (whooping cough) and Bordetella parapertussis (whooping cough-like syndrome).
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