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Factor XI Activity
MessageIf the patient's hematocrit exceeds 55%, the volume of citrate in the collection tube must be adjusted. Refer to Coagulation Collection Procedures for directions.
Test Code
086314
Alias/See Also
Antihemophilic Factor C; Plasma Thromboplastin Antecedent (PTA)
CPT Codes
85270
Preferred Specimen
Plasma, FROZEN
Minimum Volume
1 mL
Instructions
Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.(1) Evacuated collection tubes must be filled to completion to ensure a proper blood-to- anticoagulant ratio.(2,3) The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples unless the sample is collected using a winged (butterfly) collection system. With a winged blood collection set a discard tube should be drawn first to account for the dead space of the tubing and prevent under-filling of the evacutated tube.(4,5) When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternative anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. Print and use the Specimen Collection Bulletin as a tube-filling guide.
Transport Container
Blue-top (sodium citrate) tube
Transport Temperature
FREEZE
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis; clotted specimen; specimens that have thawed in transit; improperly labeled specimen.
Methodology
Factor XI activity is determined utilizing an aPTT-based one-stage clotting time assay. Factor XI-depleted plasma is used as the substrate and the clotting time with the patient plasma is compared to the clotting time of normal pooled plasma.
Setup Schedule
Daily 1st shift