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HSV Culture Without Typing
Test Code186072
Alias/See Also
HSV Culture; Nontyping Culture; Viral Culture, Herpes Simplex
CPT Codes
87255
Preferred Specimen
Vesicular fluid, ulcerated lesions, pharyngeal and throat swabs, urine, cerebrospinal fluid, autopsy and biopsy material, eye exudates, and vaginal swabs
Minimum Volume
Swab in viral transport medium, 1 mL fluid, 0.5 g tissue in transport medium
Instructions
Specimen is best collected within the first 3 days following the appearance of a lesion but no more than 7 days. After collection, remove the cap from the transport medium tube, and insert the swab aseptically into the transport tube and break the swab shaft. Replace the cap so that the swab will not interfere with closure and allow the tube to leak. Label the tube with patient identification and transport specimen to the laboratory at 4(degrees)C.
Transport Container
Viral, Chlamydia, or Mycoplasma culture transport provided by LabCorp, or other appropriate transport medium (for swabs); body fluids and tissue samples may be submitted in viral transport media or in a sterile leakproof container
Transport Temperature
Maintain specimen at 4(degrees)C.
Specimen Stability
72 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Bacterial swab specimen; specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additive; specimen received in expired transport media or incorrect transport devices; inappropriate specimen transport cond
Methodology
Enzyme-linked virus-inducible system (ELVIS(R)). In this cell line, HSV infection causes the expression of a beta-galactosidase "reporter gene," and it is the expression of this gene that can be used to monitor infection by HSV.
Setup Schedule
Monday - Friday 1st shift, Saturday 3rd shift