Bordetella PCR 11365X

Test Code
BORD PCR

Alias/See Also
BORD PCR

CPT Codes
87798 X 2

Includes
Whooping Cough

Preferred Specimen
Minimum: 0.3 mL of Nasopharyngeal aspirate collected in a sterile vial, or nasopharyngeal swab collected in Liquid Amies collection tube

Minimum Volume
1

Other Acceptable Specimens
Nasopharyngeal swab submitted in a V-C-M medium (green-cap) tube, or equivalent UTM or multi-microbe media (M4), or eSwabs (Liquid Amies)

Instructions
Nasopharyngeal swab collect Liquid Amies or Nasal Wash 0.3

Transport Container
VSWAB

Transport Temperature
R

Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Collection tubes with Amies gel or charcoal containing media • Samples collected using cotton or calcium alginate tipped swabs • Rigid shaft swabs

Methodology
Real-Time Polymerase Chain Reaction (PCR)

This test detects but does not distinguish between B. Pertussis and B. Holmesii.

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 1-2 days

Reference Range
B. pertussis DNA Not detected
B. parapertussis DNA Not detected


Clinical Significance
Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.