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BK Virus DNA, Quant RT-PCR
Test CodeBK VIRUS
Alias/See Also
BKVIR
CPT Codes
87799
Preferred Specimen
2 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
1
Instructions
Collect 2 lavernder EDTA Freeze plasma
Transport Container
MISC
Transport Temperature
F
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 6 months
Refrigerated: 6 days
Frozen: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Sodium heparin (green-top) tube • Lithium heparin (green-top) tube • Unspun PPT tubes • Frozen whole blood
Methodology
Real-Time Polymerase Chain Reaction (PCR)
Setup Schedule
Set up: Daily; Report available: 1 day
Reference Range
| BK Virus DNA, QN PCR | Not Detected (IU/mL) |
| BK Virus DNA, QN PCR | Not Detected (Log IU/mL) |
Clinical Significance
This test detects and quantitates BK Virus DNA in plasma specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in plasma (and urine) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 21.5-100,000,000 IU/mL (1.33-8.00 log IU/mL).
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
