BK Virus DNA PCR 11274

Test Code
BKVIR

Alias/See Also
BKVIR

CPT Codes
87799

Preferred Specimen
0.7 mL plasma, or whole blood collected in an EDTA (lavender-top), or acid citrate dextrose ACD (yellow-top) tube • Serum collected in a serum separator tube (SST®)

Minimum Volume
1

Instructions
Collect 1 lavernder EDTA Freeze plasma Lesion also

Transport Container
MISC

Transport Temperature
F

Specimen Stability
Plasma or serum
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples collected in heparin • Unspun PPT tube • Frozen whole blood • Serum collected in a no additive (red-top) tube

Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 1 day

Reference Range
<500 copies/mL

Clinical Significance
This test is used to determine the presence of BK Virus DNA in patient's specimens. Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the host's reaction to the virus. Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients. Quantitative testing may indicate change in risk over time. This is a quantitative molecular test, with a linear range of 500-500,000,000 copies/mL.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.