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HLA-B27 528

Test Code
HLA


Alias/See Also
HLA


CPT Codes
86812

Preferred Specimen
5 mL whole blood collected in sodium heparin (green-top) tube


Minimum Volume
1


Other Acceptable Specimens
Whole blood collected in: Acid citrate dextrose ACD-A or B (yellow-top), or EDTA (lavender-top) tube


Instructions
Draw one dark green tube and send whole blood at room temperature


Transport Container
DK GREEN


Transport Temperature
T


Specimen Stability
Room temperature: 7 days
Refrigerated: 5 days
Frozen: Unacceptable


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lithium heparin (green-top) tube • Clotted • Received frozen


Methodology
Flow Cytometry (FC)

Setup Schedule
Set up: Mon-Sat; Report available: 2-4 days


Reference Range

Negative



Clinical Significance
HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.


Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.