Alkaline Phosphatase, Bone Specific

Test Code

CPT Codes

Preferred Specimen
1 mL serum

Minimum Volume
0.3 mL

Other Acceptable Specimens

Serum separator tube

Allow serum samples to clot completely before centrifugation

Transport Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 60 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Gross lipemia • Non-serum samples • Grossly icteric


Reference Range
Adult Male  
18-29 Years 8.4-29.3 mcg/L
30-39 Years 7.7-21.3 mcg/L
40-49 Years 7.0-18.3 mcg/L
50-68 Years 7.6-14.9 mcg/L
Adult Female  
18-29 Years 4.7-17.8 mcg/L
30-39 Years 5.3-19.5 mcg/L
40-49 Years 5.0-18.8 mcg/L
50-76 Years 5.6-29.0 mcg/L
35-45 Years 5.0-18.2 mcg/L
Pediatric Male (mcg/mL) Female (mcg/mL)
2-24 months* 25.4-124.0 25.4-124.0
25 months-5 Years Not established Not established
6-9 Years 41.0-134.6 41.0-134.6
10-13 Years 43.8-177.4 24.2-154.2
14-17 Years 13.7-128.0 10.5-75.2
*Pediatric data from Int J Biol Markers 1996; 11:159-164.

Clinical Significance
The Bone-Specific Alkaline Phosphatase (BSAP) assay provides a general index of bone formation and a specific index of total osteoblast activity. BSAP and osteocalcin are the most effective markers of bone formation and are particularly useful for monitoring bone formation therapies and antiresorptive therapies.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.