Cryoglobulin (% Cryocrit), Serum

LAB713

Patient Preparation: Overnight fasting is required.

Specimen Type: Serum Red

Collection Container/Type: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 6 mL 

Collection Instructions: 

1. Allow serum sample to clot for one (1) hour in a 37° C water bath, oven, incubator, or heat block.


2. After clotting, centrifuge the specimen for 10 minutes at 1600 RCF(G) utilizing one of the 3 options below:
   
   
   
   1. In a "warm" centrifuge at 37° C.
   
   
   2. In a bench-top centrifuge placed in a "warm room" at 37° C.
   
   
   3. At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 1600 RCF(G).
   
   
   
   


3. After centrifuging, decant serum into a screw-cap vial and transport at room temperature.

Additional Information:

• If the sample is NOT kept at 37° C during all processing steps, the cryoprecipitate if present can precipitate out of solution and be lost in the clot or during the centrifugation process and result in a false negative result.


• DO NOT REFRIGERATE OR FREEZE.


• No tests can be added or poured off this tube; cryocrit and cryoglobulin evaluation only.
  
   

3 mL

Allow blood to clot for one (1) hour in a 37° C water bath, incubator, heat block, or heel warmer. After clotting, centrifuge the specimen as quickly as possible. It is not necessary to use a warm centrifuge but do not use a refrigerated one. Decant all of the serum into one screw-cap vial (do not aliquot any of the cryoglobulin serum into any other tube). Transport at room temperature.

Note: If the sample is not kept at 37° C during clotting, the cryoglobulin, if present, can precipitate during centrifugation and be lost in the clot resulting in a false negative result.

Room temperature: 72 hours

Refrigerated: Unacceptable

Frozen: Unacceptable

Gross hemolysis • Grossly lipemic • Icteric • Serum separator tube (SST)

5-7 days