A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

BK Virus DNA, Quantitative Real-Time PCR, Plasma

Message

Effective 06/08/2024: ​Test is being replaced by BKVG / LAB17071 Plasma, BK Virus DNA, Quantitative RT PCR performed at Inova Laboratories. 


Test Code
BK


Alias/See Also

Epic: LAB1374




CPT Codes
<p>87799</p>

Preferred Specimen

Submit only 1 of the following specimens:

 

Specimen Type: Whole Blood

Collection Container/Type

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD A or B)

Specimen Volume: 0.7 mL

Collection Instructions: 

  • Collect using standard procedures. Store samples refrigerated following collection. Do not freeze whole blood.

 

Specimen Type: Plasma EDTA

Collection Container/Type: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 mL

Collection Instructions: 

  • Collect blood in sterile tubes containing EDTA as an anticoagulant, or in Plasma Preparation Tube (PPT). Collect whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap aliquot tubes. Freeze and ship frozen.

 

Specimen Type: Serum

Collection Container/Type: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 m:

Collection Instructions: 

  • Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST ®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw-cap aliquot tubes. Freeze and ship frozen. 


Minimum Volume

0.3 mL



Instructions
Do not freeze whole blood or samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer's instructions for collection tube handling.


Transport Temperature
Frozen


Specimen Stability

Whole Blood


Room temperature: 48 hours


Refrigerated: 8 days


Frozen: Unacceptable


 


Plasma or Serum


Room temperature: 48 hours


Refrigerated: 7 days


Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Samples collected in heparin • Unspun PPT tube • Frozen whole blood • Serum collected in a no additive (red-top) tube



Methodology

Real-Time Polymerase Chain Reaction (RT-PCR) 

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
 



Setup Schedule
Set up: Daily; Report available: 1 day


Report Available

1 day



Reference Range
BK Virus DNA, QN PCRNot Detected (IU/mL)
BK Virus DNA, QN PCRNot Detected (Log IU/mL)


Clinical Significance
This test detects and quantitates BK Virus DNA in plasma specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in plasma (and urine) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 21.5-100,000,000 IU/mL (1.33-8.00 log IU/mL).


Performing Laboratory

Quest Diagnostics Nichols Institute 
14225 Newbrook Drive 
Chantilly, VA 20153




Last Updated: June 3, 2024


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.