CSF Encephalitis Antibody Evaluation with Reflex Titer and Line Blot

Follow standard CSF collection process

LAB16886 ECAB

ECAB

Initial panel consists of: Encephalitis Ab Screen Tissue IFA, AMPAR1, AMPAR2, Aquaporin 4, DPPX, GABAbR, NMDAR1, CASPAR2, LGI1, and VGKC.1) A line blot consisting of 10 analytes will be performed at an additional charge (CPT code(s): 84182 x9, 86341) as a reflex for tissue mosaic IFA suggesting one or more of the analytes on the line blot: ANNA1 (Hu), ANNA2 (Ri), PCA1 (Yo), Ma2/Ta, CV2 (CRMP5), Amphiphysin, AGNA1 (SOX1), GAD65, DNER, and Zic4. If the tissue mosaic pattern suggests ANNA3, then titer will be performed at an additional charge (CPT code(s): 86256).2) If the tissue mosaic pattern suggests PCA-2, then titer will be performed at an additional charge (CPT code(s): 86256).3) If the tissue mosaic IFA suggests PCA-Tr (DNER) and Western Blot shows DNER negative and Yo negative, then cell based assay IFA for DNER will be performed at an additional charge (CPT code(s): 86255).a. If DNER CBA is positive, then titer will be performed at an additional charge (CPT code(s): 86256).4) If the tissue mosaic IFA suggests myelin antibody, thena. Myelin antibody IFA titer will be performed at an additional charge (CPT code(s): 86256).b. Myelin Associated Glycoprotein (MAG) Antibody Western Blot (CPT code(s): 84182), in turn reflexing to MAG-SGPG and MAG ELISA for quantitation, will be performed at an additional charge (CPT code(s): 83520 x2).5) If the Mosaic CBA is positive for any given analyte (NMDAR1, AMPAR1, AMPAR2, GABA-B Receptor, LGI-1, CASPR2), and the individual CBAs DPPX and Aquaporin 4 antibody, then that analyte will be titered at an additional charge (CPT code(s): 86256 for each titer performed, 86052 for AQP4 Titer).6) If DPPX is positive, then titer will be performed at an additional charge (CPT code(s): 86256).7) If the Aquaporin 4 (NMO, neuromyelitis optica) CBA is positive, then Aquaporin 4 CBA titer will be performed at an additional charge (CPT code(s): 86052).

Minimum Testing Volume: 3.5 mL CSF Transport Temperature: Refrigerated Stability: Ambient: 24 hoursRefrigerated : 7 daysFrozen: 21 days Causes for Rejection: Received Ambient

16-Days

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