CULTURE, ACID FAST BACILLI (AFB/MYCOBACTERIA)

Alert the laboratory if Mycobacterium haemophilum, Mycobacterium ulcerans, Mycobacterium marinum, or Mycobacterium leprae is suspected Respiratory/Sputum: Collect 5 to 10 mL of respiratory specimen. Three sputum samples should be collected for acid fast smear and culture. These 3 samples should be collected at 8-24 hour intervals and include at least one first morning specimen. Submit in a sterile sealed leak proof container . Sterile Body Fluids and CSF: Submit in a sterile sealed leak proof container . Optimal volume is >5ml Urine: Obtain the entire first morning, clean-catch specimen when possible. Tissue and Biopsy Specimens: Submit in a sterile sealed leakproof container . Small biopsy samples should be moistened with a small (<1ml) amount of sterile saline for transportation Swab Samples: Submission of swab samples is strongly discouraged due to decreased recovery of Acid-Fast bacteria. Swabs are acceptable only if the specimen cannot be obtained by any other means. Specimens that leak in transit are not acceptable and will be rejected Immediate Specimen Processing: Specimens should be transported as soon as possible and must be received within 24 hours of collection (Refrigerated unless otherwise specified). For tissue specimens, a small amount of saline can be added to keep the tissue moist if necessary. Ambulatory Transport Temperature to Inova Central Lab: Refrigerated (except CSF)CSF: AmbientSpecimen Stability: 24 hours

LAB16668 CXAFB

CXAFB

Includes:Mycobacteria culture, concentration, Acid-Fast Stain.Identification will be performed at an additional charge.Susceptibilities, if indicated, will be performed at an additional charge.If appropriate, other susceptibilities can be performed at an additional charge if requested.

Processing Instructions: Specimens should be transported as soon as possible and must be received within 24 hours of collection (Refrigerated unless otherwise specified). For tissue specimens, a small amount of saline can be added to keep the tissue moist if necessary. Preferred Testing Volume: 5 mL CSF5 mL respiratory specimen1 mL body fluid40 mL urine5-10 mm tissue1 swabMinimum Testing Volume: 5 mL CSF5 mL respiratory specimen1 mL body fluid40 mL urine5-10 mm tissue1 swab Transport Temperature: Refrigerated (except CSF)CSF: Ambient CSF Stability: Ambient: 24 hoursRefrigerated : UnacceptableFrozen: UnacceptableAll Other Specimen Stability: Ambient: UnacceptableRefrigerated : 24 hoursFrozen: UnacceptableCauses for Rejection: Specimens submitted in formalin, dried swabs or dried tissue specimens, respiratory samples submitted on swabs or insufficient material submitted for the number of tests ordered. Performing Lab: Inova Central Lab

6-Weeks

1230120000