THROMBIN TIME

Collect a full tube to the fill line, following standard venipuncture techniques. Do not over or under fill tubes as the ratio of blood to anticoagulant (9: 1) is necessary for accurate results. Gently Invert the tube three or four times immediately after venipuncture to ensure proper mixing of blood and anticoagulant. 1. Draw blood into a light blue (sodium citrate) tube. Be sure to fill tubes completely. 2. Mix gently 5 to 6 times ( Do not over mix). 3. Centrifuge the sample for 10 minutes. 4. Using a pipette, aliquot plasma into a plastic vial being careful not disturb the buffy coat. 5. Centrifuge the plasma sample a second time for 10 minutes6. Using a pipette, aliquot the top portion of plasma into a plastic vial. Be careful not to disturb the sediment at the bottom (leave approx. 0. 25 mL of plasma at the bottom). 7. Label each tube as "Plasma. "Immediately freeze samplesPlatelet Poor Plasma Instructions

LAB5903 TT

TT

Processing Instructions (Lab use only): Within 1 hour of collection, centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again. Aliquot plasma into separate plastic vial leaving 0.25 mL in the bottom of centrifuged vial. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C. Minimum Testing Volume: 1 mL (plasma) Transport Temperature: Frozen Stability: Ambient: 4 hours Refrigerated : UnacceptableFrozen: (-20 °C): 2 weeks or (-70 °C): 6 months Causes for Rejection: Specimens submitted Refrigerated or greater than 4 hours after collection at room temperature, are short draws, clotted or have excessive hemolysis, icterus or lipemia.

STAT 24-Hours|Routine 24-Hours|Timed 24-Hours

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