Malaria Molecular Detection PCR, Reflex

Collect a full tube, following standard venipuncture techniques.

LAB16302 LCMAL

LCMAL

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Processing Instructions (Lab use only): Both blood and slides are requiredSend specimen in original tube along with 2 thin and 2 thick blood films1. Ideally, blood films should be made directly from uncoagulated blood acquired via fingerstick. However, EDTA anticoagulated blood is also acceptable.2. Prepare thin blood films as follows: a. Prepare a thin film with a "feathered edge" that is no more than a single cell thick. b. Allow the film to thoroughly air dry and then fix by briefly immersing in either absolute or 95% methyl alcohol. c. Allow to air dry after fixation.3. Prepare thick blood films as follows: a. Place a large drop of blood (approximately the size of a dime and preferably from a fingerstick) on a slide. b. Using a corner of a second slide, spread the drop in a circular motion while applying firm pressure to literally scratch the blood onto the carrier slide. This technique allows the blood to dry quickly and adhere well to the slide. Use approximately 20 circular sweeps with the second slide. The drop of blood should be about the size of a quarter when finished. c. Do not fix. Air dry thoroughly (approximately 45 minutes) before placing in transport container . Minimum Testing Volume: 1.0 mL Whole Blood Transport Temperature: Refrigerated Stability: Ambient: 7 daysRefrigerated : 7 daysFrozen: N/A Causes for Rejection: Gross hemolysisGross lipemia

4-Days

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