Paraneoplastic Antibody Expanded Evaluation with Reflex to Titer and Line Blot

Collection Instructions: Collect 4 full tubes in order to obtain the preferred amount of serum. Immediate Specimen Processing: Centrifuge specimen before transporting to the lab. If drawn using a plain red-top tube, transfer serum to a plastic screw-cap tube prior to transportation. Submit all four (4) container s to the main lab Refrigerated Ambulatory Transport Temperature to Inova Central Lab: Refrigerated Specimen Stability: 14 days

LAB16274 PNEVE

PNEVE

Panel includes: Initial panel consits of:Paraneoplastic Expanded Ab Screen Tissue IFA, AMPAR1, AMPAR2, Aquaporin 4, DPPX, GABAbR, NMDAR1, CASPAR2, LGI1, Ganglionic AChR, VGCC N-Type, VGCC P/Q type and VGKCIf Paraneoplastic Expanded Ab Screen, Tissue IFA suggests one or more positive analytes, a line blot with 10 analytes will be performed: ANNA1 (Hu), ANNA2 (Ri), PCA1 (Yo), Ma2/Ta, CRMP5(CV2), Amphiphysin, AGNA1 (SOX1), GAD65, PCA Tr (DNER), and Zic4.If the Paraneoplastic Expanded Ab Screen, Tissue IFA suggests ANNA3, then titer will be performed.If the Paraneoplastic Expanded Ab Screen, Tissue IFA suggests PCA-2, then titer will be performed.If the Paraneoplastic Expanded Ab Screen, Tissue IFA suggests PCA-Tr (DNER) and Neurology Antibody Line Blot shows PCA Tr (DNER) negative and PCA1 (Yo) negative, then Purkinje Cell Cytoplasmic Antibody Type Tr (DNER), CBA, IFA will be performed at an additional charge (CPT code(s): 86255).If Purkinje Cell Cytoplasmic Antibody Type Tr (DNER), CBA, IFA is positive, then titer will be performed.If the Paraneoplastic Expanded Ab Screen, Tissue IFA for Myelin antibody is positive, then:a. Myelin antibody IFA titer will be performed at an additional charge (CPT code(s): 86256).b. Myelin Associated Glycoprotein (MAG) Antibody Western Blot will be performed at an additional charge (CPT code(s): 84182)c. If Myelin Associated Glycoprotein (MAG) Antibody Western Blot is positive, then Myelin Associated Glycoprotein (MAG)-SGPG Antibody and Myelin Associated Glycoprotein (MAG) Antibody (IgM) EIA will be performed at an additional charge (CPT code(s): 83520 x2).If the Neurology Antibody, CBA is positive for NMDAR1, AMPAR2, LGI-1, or CASPR2, then titers will be performed at an additional charge (CPT code(s): 86256 for each titer performed).If the Aquaporin 4 (AQP4) Ab, CBA is positive, then titer will be performed at an additional charge (CPT code(s): 86052).If the Neurology Antibody, CBA is positive for AMPAR1 or GABA-B Receptor, then titers will be performed.If the DPPX Receptor Antibody, CBA is positive, then titer will be performed.@BKRCERMSGREFRESH(2302793)@ @BKRCERMSGREFRESH(2302794)@

Processing Instructions: Serum from all collection container s can be poured into 1 plastic screw-cap tube.Preferred Testing Volume: 8 mL serumMinimum Testing Volume: 4 mL serum Transport Temperature: Refrigerated Stability: Ambient: 24 hoursRefrigerated : 14 daysFrozen: 21 days Causes for Rejection: Gross hemolysis, Gross lipemia, Gross icterus, Turbid, Bacterial contamination, freeze/thaw greater than 3 cyclesPerforming Lab: Quest (SJC)

13-Days

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