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GI Pathogen PCR Panel
Test CodeLAB3251
Preferred Specimen
Liquid or soft stool preserved in Cary-Blair media
Minimum Volume
1 mL
Other Acceptable Specimens
Sterile container – for inpatient specimens only
Instructions
Specimens should be collected before administration of antibiotics or Barium.
Specimen Collection Procedure:
1) Use the bed pan provided to collect the specimen. Do not urinate into the pan.
2) Add enough stool to reach “FILL TO HERE” line on Cary-Blair Vial. It is very important to take samples of areas which appear bloody, mucoid or watery.
3) Recap the container and ensure that the cap is on tight.
4) Make sure that your NAME, DOB, DATE and TIME of collection is on the container.
5) Place specimen in 6X9 Biohazard zip lock bag.
6) Drop off the specimen to any patient service center.
1) Use the bed pan provided to collect the specimen. Do not urinate into the pan.
2) Add enough stool to reach “FILL TO HERE” line on Cary-Blair Vial. It is very important to take samples of areas which appear bloody, mucoid or watery.
3) Recap the container and ensure that the cap is on tight.
4) Make sure that your NAME, DOB, DATE and TIME of collection is on the container.
5) Place specimen in 6X9 Biohazard zip lock bag.
6) Drop off the specimen to any patient service center.
If stool occult blood or other tests are ordered, use a separate container for additional sample
Transport Container
Cary-Blair with Indicator (Green Cap)
Transport Temperature
Ambient
Specimen Stability
Ambient – 4 days in Cary-Blair
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Improper or no label
Improper storage and transport
Inappropriate collection such as: specimen in diaper, zip lock bag, non-preservative medium does not support enteric pathogens.
Specimen leaked in transit
Specimen >greater than 96 hours (4 Days)
Improper storage and transport
Inappropriate collection such as: specimen in diaper, zip lock bag, non-preservative medium does not support enteric pathogens.
Specimen leaked in transit
Specimen >greater than 96 hours (4 Days)
Methodology
NANOSPHERE Verigene® System utilizing polymerase chain reaction (PCR)
Setup Schedule
Daily, all shifts
Reference Range
Not detected
Clinical Significance
Detects the following organisms in stool specimens:
Campylobacter Group (comprised of C. coli, C. jejuni and C. lari)
Salmonella species
Shigella species (including S. dysenteriae, S. boydii, S. sonnie and S. flexneri)
Vibrio Group (comprised of V. cholera and V. parahaemolyticus)
Yersinia enterocolitica
Shiga Toxin 1 and 2 (STEC)
Campylobacter Group (comprised of C. coli, C. jejuni and C. lari)
Salmonella species
Shigella species (including S. dysenteriae, S. boydii, S. sonnie and S. flexneri)
Vibrio Group (comprised of V. cholera and V. parahaemolyticus)
Yersinia enterocolitica
Shiga Toxin 1 and 2 (STEC)
Performing Laboratory
Meritus Molecular Diagnostics - Microbiology
