Eye Culture with Gram Stain

Collect 2 ESwab with MiniTip from the affected area as follows:
1. Collect the swabs for culture before application of topical anesthetics because topical anesthetics possess antimicrobial activity. Take corneal scrapings after application of anesthetics.
2. Cleanse the skin around the eye with a mild antiseptic.
3. Gently remove makeup and eye ointment with sterile cotton and saline.
4. Collect specimens by swabbing.
5. Pass moistened swab 2 times over lower conjunctiva.
6. Avoid eyelid border and lashes. (Culture these areas separately in a similar fashion, if desired.)
 

For corneal scrapings for slides:
1. Scraping should only be done by an ophthalmologist.
2. Use local anesthetic and a platinum spatula.
3. Rub spatula with scrapings gently over a small area of the slide.
4. If too dry, use a very small amount of sterile water. 

Maintain sterility and forward specimens promptly at ambient temperature.