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Vitamin B1 (Thiamine), Blood, LC/MS/MSTest Code
2 mL whole blood collected in an EDTA (lavender-top) tube, protected from light, frozen immediately
Transfer whole blood to amber transport tube. If amber tube is not available, wrap tube in aluminum foil to protect from light. Freeze immediately.
Amber transport tube, light-protected
Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 35 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature and not protected from light • Received refrigerated and not protected from light
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Vitamin B1 is required for branched-chain amino acid and carbohydrate metabolism. Vitamin B1 deficiency is most often due to alcoholism or chronic illness. In the early stage, patients with vitamin B1 deficiency exhibit anorexia, irritability, apathy, and generalized weakness. Prolonged deficiency causes beriberi.
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153