Vitamin B1 (Thiamine), Blood, LC/MS/MS

Test Code
VITB1

CPT Codes
84425

Preferred Specimen
2 mL whole blood collected in an EDTA (lavender-top) tube, protected from light, frozen immediately

Minimum Volume
1 mL

Instructions
Transfer whole blood to amber transport tube. If amber tube is not available, wrap tube in aluminum foil to protect from light. Freeze immediately.

Transport Container
Amber transport tube, light-protected

Transport Temperature
Frozen

Specimen Stability
Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 35 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature and not protected from light • Received refrigerated and not protected from light

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range
Adults 78-185 nmol/L


Clinical Significance
Vitamin B1 is required for branched-chain amino acid and carbohydrate metabolism. Vitamin B1 deficiency is most often due to alcoholism or chronic illness. In the early stage, patients with vitamin B1 deficiency exhibit anorexia, irritability, apathy, and generalized weakness. Prolonged deficiency causes beriberi.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.