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Lymphocyte Subset Panel 3
MessageSpecimen is only stable for 72 hours. Do not draw before holidays or weekends. AM draws are preferred.
Test Code
LYSUB
Includes
% CD3 (Mature T Cells), Absolute CD3+ Cells, % CD4, Absolute CD4+ Cells, % CD8, Absolute CD8+ Cells, CD4/CD8 Ratio, Absolute Lymphocytes
Preferred Specimen
5 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
0.5 mL
Instructions
Maintain the specimen at ambient temperature. Do not refrigerate. It is recommended that the sample be drawn shortly before courier pick up and be received within 24 hours.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lithium heparin (green-top) tube • ACD (yellow-top) tube • Clotted
Methodology
Flow Cytometry (FC)
Setup Schedule
Set up: Daily; Report available: 2-3 days
Clinical Significance
T-lymphocyte count assists in evaluating cellular immunocompetency.
