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PTH, Intact and Calcium
Test CodePTHI
Preferred Specimen
PTH, Intact: 1.5 mL plasma collected in a EDTA (lavender-top) tube, and
Calcium: 1 mL serum
Calcium: 1 mL serum
Minimum Volume
1 mL EDTA plasma PTH, Intact • 0.5 mL serum Calcium • 1 mL serum PTH, Intact
Other Acceptable Specimens
PTH, Intact: 2 mL frozen serum
Instructions
Centrifuge and transfer plasma to a transport tube. Label as PLASMA for the PTH, Intact.
Transport Temperature
Frozen
Specimen Stability
EDTA plasma for PTH, Intact
Room temperature: 48 hours
Refrigerated: 72 hours
Frozen: 6 months
Serum for PTH, Intact
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months
Serum for Calcium
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Room temperature: 48 hours
Refrigerated: 72 hours
Frozen: 6 months
Serum for PTH, Intact
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months
Serum for Calcium
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Plasma submitted in sodium heparin (green-top) tube or lithium heparin (green-top) tube
Methodology
PTH: Immunoassay (IA) • Calcium: Spectrophotometry (SP)
Setup Schedule
Set up: Mon-Fri; Report available: 2 days
Clinical Significance
The assay is useful in making the diagnosis of primary hyperparathyroidism, secondary hyperparathyroidism, and a differential diagnosis of hypercalcemia. The assay helps in distinguishing hypercalcemia cause by either primary hyperparathyroidism or malignant disease.
