Prosigna® Breast Cancer Prognostic Gene Signature Assay

Message

ORDER AS A MISCELLANEOUS-MISC

Test Code

MISC

Quest Code

92125

Alias/See Also

PAM50

CPT Codes
81520

Preferred Specimen

Formalin-fixed, paraffin-embedded tissue block submitted in an IHC transport kit

Other Acceptable Specimens

6 unstained (minimum: 3 slides), 10-micron slides submitted in a tissue transport kit

Instructions

Surgical pathology report required.
Formalin fix (10 percent neutral buffered formalin) and paraffin embed excised tissue. Protect from excessive heat. Transport tissue block or 6 unstained 10-micron slides. (minimum: 3 slides). Transport block(s) and/or slide(s) in a tissue transport kit (recommended but not required).

Transport Temperature

Room temperature

Specimen Stability

Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Specimens fixed/processed in alternative fixatives (alcohol, Prefer) • Decalcified specimens • Needle core biopsy • Less than 10 percent tumor

Methodology
Hybridization/gene expression

Setup Schedule

Set up: Varies; Report available: 13-15 days

Reference Range

See Laboratory Report

Clinical Significance

Assess the risk of distant recurrence in post-menopausal women with early stage (stage I or stage II), hormone receptor-positive (ER+ and/or PR+) breast cancer. Useful in both node-negative and node-positive (1-3 nodes) disease.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

Last Updated: November 30, 2020

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.