Alpha Defensin, lateral Flow Assay, Synovial Fluid

Specimen Source site is required
Order as Miscellaneou test.  Testing laboratory is MAYO, Test code  ALDEF

ALDEF (MAYO)

Prosthetic joint infection
PJI
Alpha-Defensin
Synovasure

Alpha defensin

Synovial fluid in Plain red. Transport 1 mL synovial fluid in the original collection tube.  Separate sepcimens must be submitted when multiple tests are ordered.

0.5 mL
 

Anatomical source is required (eg, left knee, right shoulder, etc.).

Plastic Vial

Refrigerated

Refrigerated 7 days

Gross Hemolsis

Monday through Friday, Sunday

Same day/1day

Negative

Diagnosis of prosthetic joint infections (PJI) may be challenging in certain clinical scenarios. Multiple societies have defined criteria for establishing the presence of a PJI, including results from laboratory tests, clinical findings, and tissue histopathology. The challenge, however, is that results of these tests are frequently not available at the time of or after surgery. As an alternative, determining the cell count and differential on synovial fluid are frequently used biomarkers for PJI, however there is a lack of consensus on the optimal thresholds to use for a PJI diagnosis. Additionally, cell count and differential results require clinician interpretation as laboratories do not report abnormal levels correlating with PJI.
 
Alpha defensins are antimicrobial peptides released by activated neutrophils in response to infection and served as part of the host-defense innate immune system with broad antimicrobial activity against gram-positive and gram-negative bacteria, mycobacteria, fungi, and viruses. The presence of alpha defensins in synovial fluid may therefore be used by