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Malaria/Babesia/Other Blood Parasites
MessageThis is a time sensitvie test. Slides MUST be made within one hour of sample collection.
Test Code
MAL
Alias/See Also
Babesia Smear
Malaria-Babesia Exam
Filaria Smear
Malaria-Babesia Exam
Filaria Smear
Preferred Specimen
Smears must be prepared within 1 hour of sample collection
5 mL whole blood collected in an EDTA (lavender-top) tube
5 mL whole blood collected in an EDTA (lavender-top) tube
Patient Preparation
Blood drawn 1 to 2 hours prior to expected chill will enhance recovery of malarial parasites by the laboratory
Minimum Volume
1 mL whole blood
Instructions
Blood samples are to be taken, and slides prepared when the patient presents with symptoms of malaria, and every 6 hours for 36 hours.
Specimens obtained during the febrile state yield the greatest number of parasites in circulating blood. It is very important that at least one thick and one thin blood film smear must be submitted on two separate glass slides with one frosted end, in addition to the EDTA (lavender-top) tube.
One slide should be smeared as is done with a differential exam and one slide should have a drop of blood dried in an area about the size of a dime.
Note: Travel history (name of country and when visited) and anti-malarial agents should be noted on the requisition.
Specimens obtained during the febrile state yield the greatest number of parasites in circulating blood. It is very important that at least one thick and one thin blood film smear must be submitted on two separate glass slides with one frosted end, in addition to the EDTA (lavender-top) tube.
One slide should be smeared as is done with a differential exam and one slide should have a drop of blood dried in an area about the size of a dime.
Note: Travel history (name of country and when visited) and anti-malarial agents should be noted on the requisition.
Transport Temperature
Room temperature
Specimen Stability
Slides
Slides must be made within one hour of collection.
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Whole blood Room temperature: 24 hours Refrigerated: Unacceptable Frozen: Unacceptable
Slides must be made within one hour of collection.
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Whole blood Room temperature: 24 hours Refrigerated: Unacceptable Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis; Clotted blood; Frozen blood; Age-deteriorated blood cells (EDTA blood older than 48 hours)
Methodology
Microscopic Examination with Giemsa stain. One negative observation cannot rule out blood parasites. Babesia and other blood parasites are noted and reported.
Setup Schedule
Set up: Mon-Sat night
Report Available
Next day
Reference Range
Negative
Clinical Significance
The identification of malarial parasites and other blood parasites is used to determine treatment and prognosis. Babesia and other blood parasite are noted and reported. Microfilaria can be detected from a giemsa stain. However, this test is not recommended for the detection of microfilaria. One negative observation cannot rule out blood parasites.
Performing Laboratory
Meritus Medical Laboratory 11110 Medical Campus Rd Hagerstown, MD 21742
Last Updated: December 28, 2023