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Factor VIII Activity, Chromogenic
Test Code16049
Alias/See Also
Factor 8 Activity
CPT Codes
85240
Preferred Specimen
1 mL plasma collected in 3.2% sodium citrate (light-blue top) tube
Minimum Volume
0.5 mL
Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approx. 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Freeze immediately and ship on dry ice.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII.
Freeze immediately and ship on dry ice.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 8 hours
Refrigerated: 8 hours
Frozen: 21 days
Refrigerated: 8 hours
Frozen: 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature • Received refrigerated • Received thawed • Hemolysis
Methodology
Chromogenic Assay
Setup Schedule
Set up: Wed, Sat a.m.; Report available: 2-6 days
Reference Range
50-160 % normal
Clinical Significance
Preferred test in patients with Lupus anticoagulant, on heparin or direct thrombin inhibitors, or on Factor VIII concentrates.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
Last Updated: March 17, 2023
