| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Mycobacterium tuberculosis Complex and Rifampin Resistance, PCR, Non-Sputum
Test CodeMBTDD-REF
Quest Code
36925
CPT Codes
87801
Preferred Specimen
5 mL bronchial brush/wash, tracheal aspirate or urine collected in a sterile, leak-proof container
Minimum Volume
3 mL bronchial brush/wash, tracheal aspirate or urine
0.5 mL body fluid, CSF, or tissue
0.5 mL body fluid, CSF, or tissue
Other Acceptable Specimens
1 mL body fluid, or CSF, or tissue collected in a sterile, leak-proof container
Instructions
For tissue, add a small amount of sterile, non-bacteriostatic saline to container (no fixative or preservative).
For sputum, please order Test Code 94577.
For sputum, please order Test Code 94577.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Sputum • Specimen received in formalin and/or alcohol • Processed/concentrated specimen from client
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
The analytical performance characteristics of this assay have been determined by Quest Diagnostics. The modifications have not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: Next day
Reference Range
| MTB Complex | Not detected |
| Rifampin Resistance | Not detected |
Clinical Significance
Globally, about 2 billion people are infected with MTB. In 2010, 8.8 million people developed active disease and 1.4 million people lost their lives to the illness. There were 9951 new cases of tuberculosis reported in the United States in 2012 (a rate of 3.2 cases per 100,000). In 2011, 536 deaths were attributed to tuberculosis infections.
Standard treatment regimens for tuberculosis involve prolonged administration of multiple drugs and is usually highly effective. However, MTB-complex strains that are resistant to one or more of first line drugs require individualized treatment. Rifampin resistance is often an indication of multidrug resistance to tuberculosis (MDR TB), which is defined as resistance to at least rifampin (RIF) and isoniazid (INH). In the Unites States overall RIF resistance is approximately 1.8% with approximately 90% of these strains resistant to at least RIF and INH.
Standard treatment regimens for tuberculosis involve prolonged administration of multiple drugs and is usually highly effective. However, MTB-complex strains that are resistant to one or more of first line drugs require individualized treatment. Rifampin resistance is often an indication of multidrug resistance to tuberculosis (MDR TB), which is defined as resistance to at least rifampin (RIF) and isoniazid (INH). In the Unites States overall RIF resistance is approximately 1.8% with approximately 90% of these strains resistant to at least RIF and INH.
Performing Laboratory
Quest Diagnostics Nichols Institute Chantilly
14225 Newbrook Dr
Chantilly, VA 20153
Last Updated: July 22, 2019
