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Cytology Buccal Mucosa
MessageSubmit specimen with a Pathology requisition.
Label specimen container with patient's complete first and last name, date of birth, date and time of collection, and specimen source.
Label specimen container with patient's complete first and last name, date of birth, date and time of collection, and specimen source.
Test Code
AP005
Preferred Specimen
•Send the specimen to the Laboratory in an appropriate closed, labeled container with the appropriate fixative for the tests requested. If you are unsure of the proper fixative, contact the Histology laboratory at extension 3405 for clarification. • Specimen source If submitting multiple specimens, the label must match the requisition as A, B, C, D, etc. Label specimen container with patient's complete first and last name, date of birth, date and time of collection, and specimen source. Optimally, the specimens should be submitted to the laboratory unfixed or placed in Cytolyt fixative. After collection, the material must be sent to the laboratory as soon as possible. Cellular autolysis begins immediately after the sample is collected (e.g., urine or bloody serous effusion). Collection of each type of specimen is presented here for correct procedure. Diagnostic cytology is based on three basic sampling techniques: (1) collection of exfoliated cells, (2) collection of cells removed by brushing or similar abrasive techniques, and (3) aspiration biopsy or removal of cells from non-surface bearing tissues by means of a needle, with or without a syringe. If there is an unavoidable delay encountered in the delivery of the specimens (e.g., weekend, etc), specimens must be refrigerated or preserved with Cytolyt fixative. Specimens can be refrigerated for a week or more and still be satisfactory. DO NOT freeze the specimens. Freezing will fracture cells.
Instructions
1.) Sex Chromatin Counts A. Saline Cell Suspension 1. Ask patient to rinse mouth with water. 2. Draw angle of mouth laterally with little finger of one hand. 3. Firmly draw tongue depressor along buccal reflex collecting "milky " cellular fluid. 4. Place fluid in approximately 10 ml of physiologic saline and shake gently. 5. Label container with patient's name, date of birth, and date of collection. 6. Send specimen along with completed requisition to the laboratory. B. Direct Smears 1. Ask patient to rinse mouth with water. 2. Label several clean frosted-end slides with patient's name, date of collection, and site of specimen. 3. Open bottle of fixative (95% alcohol). 4. Hold one glass slide between thumb and forefinger. 5. Draw angle of mouth laterally with little finger of the same hand. 6. Draw tongue depressor along buccal reflex collecting "milky" cellular fluid. 7. Quickly smear "milky" fluid on slide and immediately drop smear into fixative. 8. Send specimen and completed requisition form to Laboratory. 2.)A. If the lesion is moist 1. Label several clean frosted-end slides with patient's name, date of collection, and site of specimen. 2. Open bottle of fixative (95% alcohol). 3. Scrape the lesion with a tongue depressor and smear the material onto the slide. 4. Immediately drop the slide into fixative. 5. Send specimen and completed requisition form to the Laboratory. B. If the lesion is dry or appears necrotic 1. Label several clean frosted-end slides with patient's name, date of collection, and site of specimen. 2. Open bottle of fixative (95% alcohol). 3. Gently moisten lesion and remove the necrotic debris with a non-absorbent cotton swab, which has been dipped in saline. 4. Discard swab and debris. 5. Using a second cotton swab moistened with saline, gently rub the advancing margins of the lesion. 6. Quickly roll the swab on a glass slide and immediately drop slide into fixative. 7. Send specimen and completed requisition form to the Laboratory. For Cancer Detection
Last Updated: May 13, 2020