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DIRECT COOMBS PANEL
MessageMonospecific coombs is used for testing specimens from adult patients. Polyspecific coombs is normally used to test samples obtained from infants that are less than six months of age.
Test Code
COOMBSP
CPT Codes
86880 X 2
Includes
IgG coombs
ANTI-C3b and Anti-C3d
ANTI-C3b and Anti-C3d
Preferred Specimen
Whole Blood: 3 mL EDTA
Other Acceptable Specimens
Cord blood
Specimen Stability
Room Temperature: Do not store at room temperature
Refrigerated: 2-8o C for up to 72 hours
Frozen: Do not freeze
Refrigerated: 2-8o C for up to 72 hours
Frozen: Do not freeze
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples not labeled with complete first and last name and date of birth
Hemolyzed samples
Hemolyzed samples
Methodology
Agglutination
Setup Schedule
Daily
Report Available
Same Day
Reference Range
Negative
Clinical Significance
The Direct Antiglobulin Test (DAT) is used to detect in vivo sensitization of red blood cells. This sensitization may or may not be associated with shortened red cell survival. Red cells can become coated with complement because red cell antigen-antibody interactions or immune complexes have activated the complement cascade. However complement coating does not lead to a visible agglutination reaction. C3 and C4 are the complement components detected most readily on the red cell. Of these two, C3 is the most clinically significant. Complement sensitization can be detected when an antiglobulin reagent containing antibodies to C3b/C3d is used.
The following are some possible causes of a positive DAT:
1. Patient cells are coated with autoantibodies.
2. Donor cells in patient's circulation are coated with alloantibodies.
3. Alloantibodies in donor plasma coating patient cells.
4. Maternal alloantibodies crossing the placenta to coat incompatible fetal cells.
5. Various drug interactions.
The following are some possible causes of a positive DAT:
1. Patient cells are coated with autoantibodies.
2. Donor cells in patient's circulation are coated with alloantibodies.
3. Alloantibodies in donor plasma coating patient cells.
4. Maternal alloantibodies crossing the placenta to coat incompatible fetal cells.
5. Various drug interactions.
Performing Laboratory
Frederick Health Laboratory
400 W 7th St
Frederick, MD 21701
Last Updated: April 8, 2022