VITAMIN D 25 HYDROXY TOTAL

Test Code

VIT D25

Alias/See Also

VD25TOTAL
VD25

CPT Codes
82306

Preferred Specimen

Lithium heparin (PST)

Minimum Volume

1.0 mL

Other Acceptable Specimens

Red top tube
SST (gold) tube
Tiger top tube
EDTA Lavender

Specimen Stability

Room Temperature: 8 hours at 20-25 deg C
Refrigerator: 4 days at 2-8 deg C
Frozen: 24 weeks at -20C, Freeze only once

Methodology
Chemiluminescent

Setup Schedule

Daily

Report Available

Same day

Reference Range

Deficiency <10 ng/mL
Insufficiency 10-30 ng/mL
Sufficiency 30-100 ng/mL
Toxicity >100 ng/m

Performing Laboratory
Frederick Health Laboratory

Last Updated: September 18, 2025

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.