Aldosterone, LC/MS/MS

Test Code

CPT Codes

Preferred Specimen
1 mL serum collected in a red-top tube (no gel)

Minimum Volume
0.25 mL

Other Acceptable Specimens
Plasma collected in: EDTA (lavender-top), sodium heparin (green-top), or lithium heparin (green-top)

Separate serum after clotting. Do not submit glass tubes.
Draw "upright" samples at least 1/2 hour after patient sits up.

Transport Container
Transport tube

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Moderate to gross hemolysis • Serum separator tube (SST)

Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Report Available
3-7 days

Aldosterone concentration varies based on body position and sodium intake.  24-hour urine specimens for aldosterone are an alternative.

Reference Range
Upright 8:00-10:00 am≤28 ng/dL
Upright 4:00-6:00 pm≤21 ng/dL
Supine 8:00-10:00 am3-16 ng/dL
1-12 months**2-70 ng/dL
1-4 years**2-37 ng/dL
5-9 years≤9 ng/dL
10-13 years≤21 ng/dL
14-17 years≤35 ng/dL
Premature (31-35 weeks)**≤144 ng/dL
Term**≤217 ng/dL
Tanner Stages** 
II-III Males1-13 ng/dL
II-III Females2-20 ng/dL
IV-V Males3-14 ng/dL
IV-V Females4-32 ng/dL

**Pediatric data from J Clin Endocrinol Metab. 1992;75:1491 and J CLin Endocrinol Metab. 1989; 69;1133-1136.

Clinical Significance
Approximately 1-2% of individuals with primary hypertension have primary hyperaldosteronism characterized by hypokalemia (low potassium) and low direct renin. Because serum aldosterone concentrations vary due to dietary sodium intake and body positions, some physicians prefer measurement of 24-hour urine concentrations for aldosterone.

Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.