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Culture, Virus, Winter Respiratory (WRVS)
Test Code426
CPT Codes
87300-Tissue culture; 87260-Adenovirus (if appropriate); 87275-Influenza A virus (if appropriate); 87276-Influenza B virus (if appropriate); 87279 x3- Parainfluenza (in appropriate); 87280-Respiratory Syncytial Virus (if appropriate)
Preferred Specimen
Specimen submitted for winter respiratory viral culture should be collected from appropriate site early in course of infection as follows:
Nasopharyngeal Swab
Container/Tube: Viral transport medium-Throat swab and bacterial culture swab is not acceptable.
Specimen Volume: Swab
Collection Instructions: 1. Vigorously collect nasopharyngeal specimen using a sterile, cotton swab provided with viral transport media. 2. Place swab in vial of viral transport medium. 3. Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Respiratory Fluid Bronchoscopy
Container/Tube: Viral transport medium-Bacterial culture swab is not acceptable.
Specimen Volume: <0.5 mL of bronchoscopy fluid
Collection Instructions: Label vial with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Nasopharyngeal Wash
Container/Tube: Culture transport container or viral transport medium-Bacterial culture swab is not acceptable.
Specimen Volume: 0.5 mL nasopharyngeal wash
Collection Instructions: Label container/vial with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Tissue
Container/Tube: Viral transport medium
Specimen Volume: 0.5 g of tissue
Collection Instructions: Label vial with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Nasopharyngeal Swab
Container/Tube: Viral transport medium-Throat swab and bacterial culture swab is not acceptable.
Specimen Volume: Swab
Collection Instructions: 1. Vigorously collect nasopharyngeal specimen using a sterile, cotton swab provided with viral transport media. 2. Place swab in vial of viral transport medium. 3. Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Respiratory Fluid Bronchoscopy
Container/Tube: Viral transport medium-Bacterial culture swab is not acceptable.
Specimen Volume: <0.5 mL of bronchoscopy fluid
Collection Instructions: Label vial with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Nasopharyngeal Wash
Container/Tube: Culture transport container or viral transport medium-Bacterial culture swab is not acceptable.
Specimen Volume: 0.5 mL nasopharyngeal wash
Collection Instructions: Label container/vial with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Tissue
Container/Tube: Viral transport medium
Specimen Volume: 0.5 g of tissue
Collection Instructions: Label vial with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Include a brief clinical history and virus suspected.
Transport Temperature
Refrigerate
Setup Schedule
Monday through Sunday (December-April)
Reference Range
No virus isolated
Clinical Significance
Useful for the isolation and identification of 7 respiratory viral pathogens (adenovirus, influenza A and B virus, parainfluenza 1, 2, and 3 virus, and respiratory syncytial virus) from clinical specimens. Other viruses such as herpes simplex virus, rhinoviruses, and cytomegalovirus are also frequently isolated from these specimens during the winter respiratory season.
Performed By
CoxHealth
