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Cytomegalovirus (CMV) Early Nuclear Antigen Detection (ENAD) and Culture
Message Shell vial assay will be performed by IFA within 24 to 72 hours of specimen arrival and culture tubes will be inoculated.
Test Code
454
CPT Codes
87252-Virus identification, tissue culture inoculation and observation; 87254-Virus centrifuge enhanced technique
Preferred Specimen
Specimen submitted for CMV isolation and identification should be collected from appropriate site early in course of the infection as follows:
Fluid
Container/Tube: Sterile vial(s) in viral transport medium
Specimen Volume: 1 mL of fluid (bronchoscopy, brush, amniotic fluid, nasopharyngeal wash, etc.)
Collection Instructions: Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Lesion, Dermal, or Tissue
Container/Tube: Sterile vial(s) in viral transport medium
Specimen Volume: 0.5 g of specimen
Collection Instructions: Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Spinal Fluid
Container/Tube: Sterile vial(s)
Specimen Volume: 1 mL of spinal fluid
Collection Instructions: Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Swab
Container/Tube: Sterile vial(s) in viral transport medium
Specimen Volume: Swab
Collection Instructions: 1. Vigorously rub collection site. 2. Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Urine
Container/Tube: Sterile container(s)
Specimen Volume: 1 mL of urine
Collection Instructions: Label container with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Fluid
Container/Tube: Sterile vial(s) in viral transport medium
Specimen Volume: 1 mL of fluid (bronchoscopy, brush, amniotic fluid, nasopharyngeal wash, etc.)
Collection Instructions: Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Lesion, Dermal, or Tissue
Container/Tube: Sterile vial(s) in viral transport medium
Specimen Volume: 0.5 g of specimen
Collection Instructions: Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Spinal Fluid
Container/Tube: Sterile vial(s)
Specimen Volume: 1 mL of spinal fluid
Collection Instructions: Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Swab
Container/Tube: Sterile vial(s) in viral transport medium
Specimen Volume: Swab
Collection Instructions: 1. Vigorously rub collection site. 2. Label vial with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Urine
Container/Tube: Sterile container(s)
Specimen Volume: 1 mL of urine
Collection Instructions: Label container with patient’s full name (first and last), date and actual time of collection, and type of specimen.
Note: 1. Specimen source is required. 2. Include a brief clinical history.
Transport Temperature
Refrigerate
Methodology
Culture/Immunofluorescence Assay (IFA)
Setup Schedule
Monday through Sunday
Reference Range
CULTURE
Negative: no virus isolated
Positive: positive for CMV
ENAD
Negative: negative
Positive: positive
Critical value (automatic call-back): all positives
Negative: no virus isolated
Positive: positive for CMV
ENAD
Negative: negative
Positive: positive
Critical value (automatic call-back): all positives
Clinical Significance
Useful for the isolation and identification of CMV during primary or chronic infections or during periods of viral reactivation from clinical specimens.
Performed By
CoxHealth
