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Pneumocystis carinii Preparation
Test Code3944433
CPT Codes
88312
Preferred Specimen
Submit only 1 of the following specimens:
Note: Pneumocystis preparations applied to expectorated sputum have an extremely low yield and will not be done without consultation with a pathologist.
Bronchial Washing
Container/Tube: Sterile container(s)
Specimen Volume: Bronchial washing
Forms: Cytology Request Form
Collection Instructions: Label container with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Inadequate specimen will not be tested.
Lung Biopsy
Container/Tube: Sterile container(s)-Specimen sent in 95% alcohol is also acceptable.
Specimen Volume: Lung biopsy
Forms: Cytology Request Form
Collection Instructions: Label container with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Inadequate specimen will not be tested.
Transthoracic Needle Aspirate
Container/Tube: Slide(s)
Forms: Cytology Request Form
Collection Instructions: 1. When placing needle aspirate on slide, single drops should be applied and allowed to air dry without spreading. 2. Label slide with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. The following specimens will not be tested: A. Insufficient fluid volume B. Smeared slide
Note: Pneumocystis preparations applied to expectorated sputum have an extremely low yield and will not be done without consultation with a pathologist.
Bronchial Washing
Container/Tube: Sterile container(s)
Specimen Volume: Bronchial washing
Forms: Cytology Request Form
Collection Instructions: Label container with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Inadequate specimen will not be tested.
Lung Biopsy
Container/Tube: Sterile container(s)-Specimen sent in 95% alcohol is also acceptable.
Specimen Volume: Lung biopsy
Forms: Cytology Request Form
Collection Instructions: Label container with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. Inadequate specimen will not be tested.
Transthoracic Needle Aspirate
Container/Tube: Slide(s)
Forms: Cytology Request Form
Collection Instructions: 1. When placing needle aspirate on slide, single drops should be applied and allowed to air dry without spreading. 2. Label slide with patient’s name (first and last) and date and actual time of collection.
Note: 1. Specimen source is required. 2. The following specimens will not be tested: A. Insufficient fluid volume B. Smeared slide
Transport Temperature
Refrigerate
Methodology
Gomori’s Methenamine Silver Stain Includes methenamine sliver stain. Biopsy will have routine gross and microscopic description.
Setup Schedule
Monday through Saturday
Reference Range
No Pneumocystis identified
Clinical Significance
Useful for diagnosis of Pneumocystis carinii pneumonia.
Patients with clinical diffuse interstitial pneumonitis and/or immunocompromised patients have a high incidence of infection with Pneumocystis carinii (as high as 44% in some series). There is evidence that suspension smear preparations are superior to touch preparations (of biopsy specimens) for rapid diagnosis. Pneumocystis carinii pneumonia may be rapidly progressive and life-threatening so that rapid diagnosis is important to allow prompt institution of therapy. Pneumocystis carinii is the most frequent cause of death in children with acute lymphocytic leukemia in remission resulting in some institutions routinely giving prophylactic trimethoprim-sulfamethoxazole to their leukemic children undergoing antineoplastic therapy.
Patients with clinical diffuse interstitial pneumonitis and/or immunocompromised patients have a high incidence of infection with Pneumocystis carinii (as high as 44% in some series). There is evidence that suspension smear preparations are superior to touch preparations (of biopsy specimens) for rapid diagnosis. Pneumocystis carinii pneumonia may be rapidly progressive and life-threatening so that rapid diagnosis is important to allow prompt institution of therapy. Pneumocystis carinii is the most frequent cause of death in children with acute lymphocytic leukemia in remission resulting in some institutions routinely giving prophylactic trimethoprim-sulfamethoxazole to their leukemic children undergoing antineoplastic therapy.
Performed By
CoxHealth
