Factor VIII, Plasma

Message

Unspun specimens must arrive at the Lab within 30 minutes at Room Temp for correct processing.
Off campus sites must send Platelet Poor frozen plasma to Cox South Laboratory.

Test Code

CPT Codes
85240

Preferred Specimen

2mL frozen plama
Container/Tube: Light blue-top (citrate) tube-
Specimen Volume: Full tube
Collection Instructions: Blood to anticoagulant ratio is critical.
Note: 1. Results may be affected by hematocrit >55%.

Minimum Volume

2 ml plasma

Instructions

Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 mins of collection. Use a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Freeze immediately and ship on dry ice.

Transport Temperature

Frozen

Specimen Stability

Frozen: 14 days.
Unspun specimen must arrive within 30 minutes of draw or send platelet poor frozen plasma.

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Grossly hemolyzed, clotted blood, or an insufficient specimen is not acceptable.

Methodology
Partial Thromboplastin Time-Based Clotting Method/Standard Curve from Reference Plasma

Setup Schedule

Monday through Sunday

Reference Range

40-150% activity compared to normal plasma pool

Clinical Significance

Useful for evaluating and monitoring hemophilia A and von Willebrand disease.

Performed By
CoxHealth

Performing Laboratory
Cox Health South Lab

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.