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Protein Electrophoresis with Pathologist Interpretation, Serum or Urine
Test Code3951122
CPT Codes
84165-Electrophoresis, protein; 86334-Immunofixation electrophoresis, protein, serum (if appropriate); 86335-Immunofixation electrophoresis, protein, urine (if appropriate)
Includes
Includes protein, total with electrophoresis for quantitation of albumin, alpha-1, alpha-2, beta- and gamma globulins, and pathologist’s comments. If monoclonal gammopathy is detected, #717 Immunofixation Electrophoresis with Pathologist Interpretation, Serum or Urine will be performed and charged separately (unless monoclonal gammaglobulin has been previously identified and on record).
Preferred Specimen
Submit only 1 of the following specimens:
Serum
Container/Tube: Serum gel tube(s)
Specimen Volume: 3 mL (minimum volume: 1 mL) of serum
Collection Instructions: Avoid hemolysis.
Note: 1. Indicate serum. 2. Label specimen appropriately (serum).
Urine, Random Collection
Container/Tube: Plastic urine container(s)
Specimen Volume: 25 mL from a random urine collection
Collection Instructions: No preservative. Centrifuge and/or filter urines.
Note: 1. Indicate random urine 2. Label specimen appropriately (random urine).
Urine, 24-Hour Collection
Container/Tube: Plastic urine container(s)
Specimen Volume: 25 mL from a 24-hour urine collection
Collection Instructions: No preservative. Centrifuge and/or filter urines.
Note: 1. 24-Hour volume is required. 2. Follow instructions in Urine Collection in Special Instructions. 3. Indicate 24-hour urine. 4. Label specimen appropriately (24-hour urine).
Serum
Container/Tube: Serum gel tube(s)
Specimen Volume: 3 mL (minimum volume: 1 mL) of serum
Collection Instructions: Avoid hemolysis.
Note: 1. Indicate serum. 2. Label specimen appropriately (serum).
Urine, Random Collection
Container/Tube: Plastic urine container(s)
Specimen Volume: 25 mL from a random urine collection
Collection Instructions: No preservative. Centrifuge and/or filter urines.
Note: 1. Indicate random urine 2. Label specimen appropriately (random urine).
Urine, 24-Hour Collection
Container/Tube: Plastic urine container(s)
Specimen Volume: 25 mL from a 24-hour urine collection
Collection Instructions: No preservative. Centrifuge and/or filter urines.
Note: 1. 24-Hour volume is required. 2. Follow instructions in Urine Collection in Special Instructions. 3. Indicate 24-hour urine. 4. Label specimen appropriately (24-hour urine).
Transport Temperature
Refrigerate
Methodology
Agarose Gel Electrophoresis
Setup Schedule
Monday through Friday
Reference Range
SERUM
Protein total: 6.4-8.5 g/dL
Albumin: 3.5-5.2 g/dL
Alpha-1-globulin: 0.1-0.3 g/dL
Alpha-2-globulin: 0.6-1.1 g/dL
Beta-globulin: 0.5-1.1 g/dL
Gamma-globulin: 0.5-1.4 g/dL
URINE
An interpretive report will be provided.
Protein total: 6.4-8.5 g/dL
Albumin: 3.5-5.2 g/dL
Alpha-1-globulin: 0.1-0.3 g/dL
Alpha-2-globulin: 0.6-1.1 g/dL
Beta-globulin: 0.5-1.1 g/dL
Gamma-globulin: 0.5-1.4 g/dL
URINE
An interpretive report will be provided.
Clinical Significance
This test is primarily used for detection of monoclonal gammopathies, low gamma globulins, and abnormalities in the other normally occurring protein patterns; for evaluation of serum protein evaluation, nutritional status, and diffuse polyclonal gamma globulin; and in the work-up for liver disease.
Performed By
CoxHealth