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Amylase Isoenzymes with Reflex to Macroamylase [36187]
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Test Code
AMYISO
Alias/See Also
36187
CPT Codes
82150 (x2)
Includes
Amylase, Pancreatic Isoenzyme, Salivary Isoenzymes (calculated)
If Amylase Isoenzymes are >115 U/L, then Macroamylase will be performed at an additional charge (CPT code(s): 82150).
If Amylase Isoenzymes are >115 U/L, then Macroamylase will be performed at an additional charge (CPT code(s): 82150).
Preferred Specimen
2 mL serum
Minimum Volume
1 mL
Transport Container
Plastic screw-cap vial
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature = Unacceptable
Refrigerated = 7 days
Frozen = 30 days
Refrigerated = 7 days
Frozen = 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis
Methodology
Polyethylene Glycol Precipitation
Spectrophotometry
Setup Schedule
Set up: Sun-Fri; Report available: Next day
Limitations
Oxalate, citrate, and lipemic sera can decrease results.
Reference Range
Amylase | 21-101 U/L |
Pancreatic Isoenzyme | 16-46 U/L |
Salivary Isoenzymes | 4-61 U/L |
Clinical Significance
Amylase is produced primarily in the pancreas and salivary glands. Isoenzymes may be used to determine the source of an elevated amylase concentration. Macroamylase is a laboratory and not a clinical entity. Amylase can complex with immunoglobulins or other high molecular weight proteins and serum amylase will appear falsely elevated. No clinical symptoms are associated with macroamylasemia.
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |
Last Updated: November 24, 2021