Cortisol, Free, 24-Hour Urine with Creatinine, LC/MS/MS

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Test Code

CORTFU

CPT Codes
82530

Includes

CPT code 81050 may be added at an additional charge for volume measurement

Preferred Specimen

2 mL of a 24-hour urine collected in a leak-proof urine container, no preservative

Minimum Volume

0.5 mL

Other Acceptable Specimens

24-hour urine in 25 mL 6N HCl, or 10 mL concentrated glacial acetic acid, or in 10 grams boric acid, submitted in a leak-proof urine container

Instructions

Record 24-hour urine volume on test requisition and urine vial. Reference ranges do not apply to random urine samples.

Transport Container

Urine Container, leak-proof

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 14 days
Refrigerated: 21 days
Frozen: 90 days

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Monday-Friday Night
Report available: 3 Days

Reference Range

Cortisol, Free, 24-Hr Urine

Adult	4.0-50.0
Pediatric
1-4 Years	0.9-8.2 mcg/24 hrs
5-9 Years	1.0-30.0 mcg/24 hrs
10-13 Years	1.0-45.0 mcg/24 hrs
14-17 Years	3.0-55.0 mcg/24 hrs

Creatinine, 24-Hour Urine

<3 Years	Not established
3-8 Years	0.10-0.80 g/24 hours
9-12 Years	0.20-1.40 g/24 hours
13-17 Years	0.40-1.90 g/24 hours
>17 Years	0.50-2.15 g/24 hours

Clinical Significance

Urinary Free Cortisol is useful in the detection of patients with Cushing's syndrome for whom Free Cortisol concentrations are elevated.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

Last Updated: November 29, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.